cell adhesion assay - using collagen (Jun/16/2010 )
Hi,
I'm currently oerforming cell adhesion assays on my cell lines expressing my gene of interest vs cell line transfected with vector only. I'm using a colorimetric method. Howver, I do not understand the rationale behind this assay. If a cell line is tumor suppressive, do we expect it to have more cells adhering, i.e higher abosrbance reading comapred to the control or vice versa?
I thought if a cell line is tumor suppresisve, more cells would adhere, demonstrating reduced metastasis ability?
-SF_HK-
SF_HK on Jun 17 2010, 02:26 AM said:
Hi,
I'm currently oerforming cell adhesion assays on my cell lines expressing my gene of interest vs cell line transfected with vector only. I'm using a colorimetric method. Howver, I do not understand the rationale behind this assay. If a cell line is tumor suppressive, do we expect it to have more cells adhering, i.e higher abosrbance reading comapred to the control or vice versa?
I thought if a cell line is tumor suppresisve, more cells would adhere, demonstrating reduced metastasis ability?
I'm currently oerforming cell adhesion assays on my cell lines expressing my gene of interest vs cell line transfected with vector only. I'm using a colorimetric method. Howver, I do not understand the rationale behind this assay. If a cell line is tumor suppressive, do we expect it to have more cells adhering, i.e higher abosrbance reading comapred to the control or vice versa?
I thought if a cell line is tumor suppresisve, more cells would adhere, demonstrating reduced metastasis ability?
this may be a feature of carcinoma or adenocarcinoma cells: Attachment gives survival signals which are less needed for carcinoma cells; perform also as a control experiment a poly-HEMA attachment assay
-Inmost sun-