total RNA yield - (May/27/2010 )

Hi,

I am confused . My total RNA yield is increased in my experimental group. Does this mean that the experimental treatment is possibly increasing RNA and protein synthesis?

Would you like to explain a little about the background of your experiment. wat kind of treatment you are giving and what possible outcome you are expecting

It might just be an indication of higher cell numbers or a better extraction. What are you extracting RNA from - tissue or cells?

Hi,

I am extracting RNA from liver tissue. Insulin resistant rats were treated with a compound that improves insulin sensitivity. Insulin stimulates protein synthesis, thus I thought this could possibly explain the increased RNA yield in my treated samples. I used the same weight of tissue and the same RNA extraction protocol for all samples. I do not think it could be due to RNA extraction procedure. The increased RNA yield in treated samples is statistically significant (p<0.0001, unpaired t-test).

Hope this helps,
Carmen