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starvation question: With o without aminoacids? - (May/24/2010 )

Hi all.

Usually I use a DMEM medium, with Fetal Calf Serum, non essencials aminoacids and glutamine. So, to starv my U87MG cells I understand that I should use DMEM free of serum, but ONLY free of serum? must I leave the aminoacids in the mixture?

Thanks in advance.

-autophagator-

autophagator on May 24 2010, 06:03 AM said:

Hi all.

Usually I use a DMEM medium, with Fetal Calf Serum, non essencials aminoacids and glutamine. So, to starv my U87MG cells I understand that I should use DMEM free of serum, but ONLY free of serum? must I leave the aminoacids in the mixture?

Thanks in advance.



Hi,

Just starve the cells without serum, that will do.

regards

jaya

-jaya2020-

Thanks for you advice Jaya.

Bst regards

A.

-autophagator-