Troubleshooting-Transfection - (May/23/2010 )

What are the possible reasons for failure of transfection experiment?
The control experiment worked fine and gave expected results.

what was different between control and treatment?

EMSA1983 on May 24 2010, 01:59 AM said:

there should be more information about the method, the cells or the gene/gene product;

my first idea is that you try to express a protein that will be toxic, and therefore, it will not be expressed or immediately eliminated; or if expressed those cells died;

another spontaneous idea is that your gene/DNA of interest was methylated at CpG islands during bacterial amplification; keyword: DNA methylation-mediated epigenetic control