Annexin V assay analysis - (May/13/2010 )
pesji on Fri Dec 17 12:20:01 2010 said:
Another possible problem could be the compensation between FL1 and FL3 (since I use Annexin V Fluos). Would you think that might create trouble ? I naively thought that FL1 and FL3 are too apart to need compensation ????????????
Replace PI by 7-ADD (ViaProbe) and be happy. This dye utilises FL-4 channel (far red), exitable by 488 and has no emission spectra overlap with FL-1.
-Denis Baev-