First time using sequencher - (May/04/2010 )

Hi, I am interested in sequencing and so I have finally got my "raw" sequencing results from DNA core. I went through sequencing through a few patients that I am interested in seqeuncher and basically the task that I want to do is look at hypermutation. So, this website I am using tells me that I need to paste an alignment but I keep on geetting an error. I don't think I am putting the right thing into the program. Program is called hypermut 2.0 Maybe, I should ask first is what is an alignment?

new2science on May 4 2010, 04:15 PM said:

Here is am image of an alignment of two DNA sequences. It was produced by ClustalW2. You need to have your DNA sequences in a text file, in FASTA format, which looks like this:

>sequence1 Everything after the first space is a comment that describes sequence1.
ACAAGATGCCATTGTCCCCCGGCCTCCTGCTGCTGCTGCTCTCCGGGGCCACGGCCACCGCTGCCCTGCC
CCTGGAGGGTGGCCCCACCGGCCGAGACAGCGAGCATATGCAGGAAGCGGCAGGAATAAGGAAAAGCAGC
CTCCTGACTTTCCTCGCTTGGTGGTTTGAGTGGACCTCCCAGGCCAGTGCCGGGCCCCTCATAGGAGAGG
AAGCTCGGGAGGTGGCCAGGCGGCAGGAAGGCGCACCCCCCCAGCAATCCGCGCGCCGGGACAGAATGCC
CTGCAGGAACTTCTTCTGGAAGACCTTCTCCTCCTGCAAATAAAACCTCACCCATGAATGCTCACGCAAG
TTTAATTACAGACCTGAA
>sequence2 The name/comment line of the sequence must begin with a ">" character
ACCGGCCGAGACAGCGAGCATATGCAGGCCTGGAGGGTGGCCCCAAGCGGCAGGAATAAGGAAAAGCAGC
ACAAGATGCCATTGTCCCCCGGCCTCCTGCTGCTGCTGCTCTCCGGGGCCACGGCCACCGCTGCCCTGCC
CCTGGAGGGTGGCCCCACCGGCCGAGACAGCGAGCATATGCAGGAAGCGGCAGGAATAAGGAAAAGCAGC
CTCCTGACTTTCCTCGCTTGGTGGTTTGAGTGGACCTCCCAGGCCAGTGCCGGGCCCCTCATAGGAGAGG
AAGCTCGGGAGGTGGCCAGGCGGCAGGAAGGCGCACCCCCCCAGCAATCCGCGCGCCGGGACAGAATGCC
CTGCAGGAACTTCTTCTGGAAGACCTTCTCCTCCTGCAAATAAAACCTCACCCATGAATGCTCACGCAAG
TCACCCATGAATGCTTTTAATTACAGACCTGAA

etc...

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