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phenol 5% help! - (Apr/28/2010 )

Hi! I would like to know how to make phenol 5% in water. It is for flagella stain
Thanks!
Analía

-analia-

w/v: take 5 gm phenol crystals and add water to 100 ml.

-mdfenko-

mdfenko on Apr 28 2010, 10:58 AM said:

w/v: take 5 gm phenol crystals and add water to 100 ml.


Do I melt phenol first?
Does it really mix? The only phenol I have ever prepare is for DNA extraction. And it becames a two-phase solution. That is why my doubts.

Thanks!
Analía

-analia-

the phenol will dissolve in the water (1gm/15ml water)

-mdfenko-