phenol 5% help! - (Apr/28/2010 )
Hi! I would like to know how to make phenol 5% in water. It is for flagella stain
Thanks!
Analía
-analia-
w/v: take 5 gm phenol crystals and add water to 100 ml.
-mdfenko-
mdfenko on Apr 28 2010, 10:58 AM said:
w/v: take 5 gm phenol crystals and add water to 100 ml.
Do I melt phenol first?
Does it really mix? The only phenol I have ever prepare is for DNA extraction. And it becames a two-phase solution. That is why my doubts.
Thanks!
Analía
-analia-
the phenol will dissolve in the water (1gm/15ml water)
-mdfenko-