KpnI Restriction enzyme - how to detect the SNP (Apr/27/2010 )

hi i am new here:
I am trying to identify this SNP: Gly16Arg of beta2 adrenergic receptor gene.
I used this primer: F: 5‚-AGCCAGTGCGCTTACCTGCCAGAC-3‚
and the reverse primer:
3‚-CATGGGTACGCGGCCTGGTGCTGCAGTGC-5‚.
Vesna D.ET,AL2002.

And The Restriction enzyme KpnI.

so how I can know the polymorphism as wild type or homo zygote SNP or hetero zygote.
thnx

I don't see any GGTACC in your primer sequence so I assume your RE site must be within your PCR product sequence. This depends how many bp your product is also. but if you digest and you see 2 or more bands on the gel compared to your original PCR product then the KpnI site must be there.

Did I help?