Alzheimer's cell model - (Apr/26/2010 )
Hi, everybody. I want to construct a cell model about Alzheimer's Disease, but I am not very familiar with this aspect. So, could you please tell me the best cel model for AD? such as which cell line is best for A-beta transfection?
Thanks so much.
CHO probably.. check out Shankar et al for the natural oligomers.. Selkoe and collaborators
forrestlin on Apr 27 2010, 03:55 AM said:
Thanks so much.
forrestlin on Tue Apr 27 02:55:51 2010 said:
Hi, everybody. I want to construct a cell model about Alzheimer's Disease, but I am not very familiar with this aspect. So, could you please tell me the best cel model for AD? such as which cell line is best for A-beta transfection?
Thanks so much.
You could use swedish mutant APP or C99+Met combinant peptide plasmid. HEK293 or HEK 293T is OK.
Good luck.
forrestlin on Tue Apr 27 02:55:51 2010 said:
Hi, everybody. I want to construct a cell model about Alzheimer's Disease, but I am not very familiar with this aspect. So, could you please tell me the best cel model for AD? such as which cell line is best for A-beta transfection?
Thanks so much.
in the past i've used HEK293T cells for Abeta transfection, and it worked well - but i'm not in that field anymore, so i don't know what the current consensus is.
people use different cell lines and make stables. HEK293, neuroblastoma (SHSY5Y) etc.
I have read journals on researchers using IMR32 cell lines for basic research studying the protective effects of plant bioactives on amyloid beta peptide insult of this cell line.
However I find this cell line a loosely adherent cell line and always detach itself from plates while changing medium even when we use collagen coated plates.
Any suggestions?
thean on Fri Jan 21 07:38:38 2011 said:
I have read journals on researchers using IMR32 cell lines for basic research studying the protective effects of plant bioactives on amyloid beta peptide insult of this cell line.
However I find this cell line a loosely adherent cell line and always detach itself from plates while changing medium even when we use collagen coated plates.
Any suggestions?
Yes, this is a known characteristic of IMR32 cells. Using warmed media helps with this, but only slightly. Treating them with BrdU or dbcAMP will cause them to differentiate and take on a neuronal morphology, and they also become more adherent.
Hi everybody!
I already evaluated the AChE and BChE inhibition capacity of different plant extracts.
Now I want to test the most promising ones in SH-SY5Y cell lines.
Apart from the cytotoxicity evaluation which assays you suggest to do to evaluate the Alzheimer's capacity?
Best regards