RT-PCR Gel - Ladder looks terrible and product is fuzzy! (Apr/21/2010 )
I'm having some issues running a gel. My product is ~80 bp so I am running it on a 2% gel at 90-100 V for 40 minutes. The ladder isn't getting good separation and my product appears fuzzy. Does anyone have any suggestions? Thanks!
-ColoSarah-
my products is 206bps,3% gel.The ladder got good separation. you may try to increase your gel concentration.try 3% gel or higher.
-spring-