Precipitation in DNAse I incubation buffer - (Apr/19/2010 )
I have a problem with the buffer of DNase I from Roche. I detected a white precipitate in the buffer. Here's the recipe for the buffer:
Incubation Buffer (10×)
400 mM Tris-HCl, 100 mM NaCl, 60 mM MgCl2, 10 mM CaCl2, pH 7.9.
Some lab partners said that sometimes that happens to the incubation buffers, and that it could be remediated with a few minutes at 37ēC, but in this case that wasn't the solution. Does someone knows what it's going on? can be remediates or it's better to throw it away?
-vabarca-
vabarca on Apr 19 2010, 05:15 PM said:
I have a problem with the buffer of DNase I from Roche. I detected a white precipitate in the buffer. Here's the recipe for the buffer:
Incubation Buffer (10×)
400 mM Tris-HCl, 100 mM NaCl, 60 mM MgCl2, 10 mM CaCl2, pH 7.9.
Some lab partners said that sometimes that happens to the incubation buffers, and that it could be remediated with a few minutes at 37ēC, but in this case that wasn't the solution. Does someone knows what it's going on? can be remediates or it's better to throw it away?
Incubation Buffer (10×)
400 mM Tris-HCl, 100 mM NaCl, 60 mM MgCl2, 10 mM CaCl2, pH 7.9.
Some lab partners said that sometimes that happens to the incubation buffers, and that it could be remediated with a few minutes at 37ēC, but in this case that wasn't the solution. Does someone knows what it's going on? can be remediates or it's better to throw it away?
I would throw it away. It's not worth it to troubleshoot and find a remedy for a buffer that can be easily re-made.
-ProteinWork-