How to Improve EC50? EC50= Half maximal effective concentration - Please please help! (Apr/18/2010 )
We did an experiment for the MEASUREMENT OF AGONIST POTENCY which investigated the responses of a section of guinea-pig ileum (mounted in an organ bath) to acetylcholine and histamine.
How can we improve/obtain more reliable values of our EC50 value, given that we had time?? How
Any clues would be great. Thanks.
-jamescorden-
jamescorden on Apr 18 2010, 11:55 AM said:
We did an experiment for the MEASUREMENT OF AGONIST POTENCY which investigated the responses of a section of guinea-pig ileum (mounted in an organ bath) to acetylcholine and histamine.
How can we improve/obtain more reliable values of our EC50 value, given that we had time?? How
Any clues would be great. Thanks.
How can we improve/obtain more reliable values of our EC50 value, given that we had time?? How
Any clues would be great. Thanks.
you have to re-done the experiment for several times, at least n = 3-times independently form each other which means using fresh preparations form other guinea pigs;
which effect of receptor stimulation are you measuring?
-Inmost sun-