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more than one band - (Apr/16/2010 )

hellow every one,

i had a problem in my results, when i do the RT-PCR for Norovirus the electrical cable of the thermal cycler was not fixed, so the reaction was stopped twice before i fix the cable tightly.

this is the picture for agarose gel electrophoresis, can any one suggest why i have more than one band?
Attached Image

-ARR-

ARR on Apr 17 2010, 03:28 AM said:

hellow every one,

i had a problem in my results, when i do the RT-PCR for Norovirus the electrical cable of the thermal cycler was not fixed, so the reaction was stopped twice before i fix the cable tightly.

this is the picture for agarose gel electrophoresis, can any one suggest why i have more than one band?


the other samples seem fine, maybe it's contamination for that one sample? I assume you mean the first lane on the left after ladder...

-chrisbelle-