IGg bands on SDS PAGE - (Apr/09/2010 )

Hi all,

After IGg purification do I have to see one band or two bands(l ight and heavey chains)?

bluebird on Apr 9 2010, 12:15 PM said:

Well, that depends whether you are running reducing or non-reducing gels. If reducing, you will see 2 bands. If you run non-reducing gels you might still see some bands corresponding to the light and heavy chains but the major band should correspond to the intact antibody.

Hope this helps.

yes it helped so much.

Many Thanks Doctor