ubiquitin isopeptidases - (Apr/09/2010 )
Hi! Do you know if there are any comercially available enzymes which could cleave ubiquitin from monoubiquitilated protein.
I've read a loy of articles but couldn't found something reasonable 
Maybe somebody knows how could I detect ubiquitination besides Western and MS?
-botanicus-
I really think you need to Western to confirm Ub. You can try a proteasome inhibotor like MG-132 to see if your protein accumulates, instead of a direct Ub ab.
MS will only map your Ub sites.
Biomol/Enzo Life Sciences makes a bunch of SENP and Ulp type enzymes.
-sumogirlie-
sumogirlie on Apr 10 2010, 04:39 AM said:
I really think you need to Western to confirm Ub. You can try a proteasome inhibotor like MG-132 to see if your protein accumulates, instead of a direct Ub ab.
MS will only map your Ub sites.
Biomol/Enzo Life Sciences makes a bunch of SENP and Ulp type enzymes.
MS will only map your Ub sites.
Biomol/Enzo Life Sciences makes a bunch of SENP and Ulp type enzymes.
thanks a lot -botanicus-