Autoclaving coated glass coverslips? - (Apr/08/2010 )
I coated my glass coverslips with PDL and gelatin, dried them, stored them in a sterile culture dish in the dark at room temperature. Now I'm worried they may be contaminated and would like to sterilize them.
Is it possible to autoclave already-coated glass coverslips?
-Sapphire.Waters-
Sapphire.Waters on Apr 8 2010, 10:23 AM said:
I coated my glass coverslips with PDL and gelatin, dried them, stored them in a sterile culture dish in the dark at room temperature. Now I'm worried they may be contaminated and would like to sterilize them.
Is it possible to autoclave already-coated glass coverslips?
Is it possible to autoclave already-coated glass coverslips?
The answer really is NO....
The best way to sterilise them would be by Gamma Irradiation....or to just re-coat some fresh coverslips
Hope this is useful
Kindest regards
Rhombus
-rhombus-
Thanks, that's what I figured. tow that I think about it, it's possible that PDL-gelatin lattices probably would've locked in the contamination I would've wanted to remove. I'm best off just making fresh coverslips.
Thanks anyway, figured it was worth a shot.
-Sapphire.Waters-