Protein Expression in stationary phase - (Mar/24/2010 )
AbhayGene on Apr 8 2010, 12:15 AM said:
OK Fishdoc...Thank you so much!
Now, my question is how long the sds-page buffer lysed sample is stable in -20?
As I was away for some reasons, I lysed the samples in sds-page buffer,cooled and placed them in -20 for more than 10 days. Can I use those samples for western blot now? are they stable enough?
Now, my question is how long the sds-page buffer lysed sample is stable in -20?
As I was away for some reasons, I lysed the samples in sds-page buffer,cooled and placed them in -20 for more than 10 days. Can I use those samples for western blot now? are they stable enough?
they should be fine. you may have to reboil. just ensure that there is no sds out of solution.
-mdfenko-
mdfenko on Apr 16 2010, 11:54 PM said:
AbhayGene on Apr 8 2010, 12:15 AM said:
OK Fishdoc...Thank you so much!
Now, my question is how long the sds-page buffer lysed sample is stable in -20?
As I was away for some reasons, I lysed the samples in sds-page buffer,cooled and placed them in -20 for more than 10 days. Can I use those samples for western blot now? are they stable enough?
Now, my question is how long the sds-page buffer lysed sample is stable in -20?
As I was away for some reasons, I lysed the samples in sds-page buffer,cooled and placed them in -20 for more than 10 days. Can I use those samples for western blot now? are they stable enough?
they should be fine. you may have to reboil. just ensure that there is no sds out of solution.
Alright. Thanks
-DNAlooper-