Illumina - Bead studio Micrarray DATA - (Mar/18/2010 )

Hi all,

Is any one working with bead studio software to analyze the illumina microarray for microRNA differential expression? Got some questions to be answered. Please let me know.

Thanks in advance.
Mr.N

-Mr. Naive-

i have used bead studio software for differential gene expression but not for microRNA. y dont u post your questions and then i'll see if i can help u..

-Helios-

Thanks for ur input Helios,

I am wondering how to compare 4 samples at a time like what we do in ANOVA (ex: A vs B, A vs C, A vs.D B vs. C, B vs. D & C vs. D). Instead the beadstudio software runs a t-test comparing each sample (say B,C,D) with a reference sample (say A).

My other question is: At what fold change can we say that the differential expression of mutant type is significantly different from that of the wild type. (ex: 2x or 3x or 10x).

3rd question: what is the difference between detection p-value and diff. p-value.

Thanks,
Naive.

Helios on Apr 8 2010, 07:52 AM said:

i have used bead studio software for differential gene expression but not for microRNA. y dont u post your questions and then i'll see if i can help u..

-Mr. Naive-