Protocol Online logo
Top : New Forum Archives (2009-): : Flow Cytometry

Rnase for flow cytometry - (Mar/17/2010 )

Hi,

I have a stock conc of 100mg/ml of Rnase. Does anyone know how much Rnase to add for cell cycle analysis? I have about 100000 cells. Also, can teh RNAse be diluted in the FACs buffer (1%FBS in PBS).

Thanks
SF

-SF_HK-

SF_HK on Mar 17 2010, 08:39 AM said:

Hi,

I have a stock conc of 100mg/ml of Rnase. Does anyone know how much Rnase to add for cell cycle analysis? I have about 100000 cells. Also, can teh RNAse be diluted in the FACs buffer (1%FBS in PBS).

Thanks
SF


I use about 100 ug/ml. I usually resuspend in 100 uL of Rnase solution

-canotto-