Rnase for flow cytometry - (Mar/17/2010 )
Hi,
I have a stock conc of 100mg/ml of Rnase. Does anyone know how much Rnase to add for cell cycle analysis? I have about 100000 cells. Also, can teh RNAse be diluted in the FACs buffer (1%FBS in PBS).
Thanks
SF
-SF_HK-
SF_HK on Mar 17 2010, 08:39 AM said:
Hi,
I have a stock conc of 100mg/ml of Rnase. Does anyone know how much Rnase to add for cell cycle analysis? I have about 100000 cells. Also, can teh RNAse be diluted in the FACs buffer (1%FBS in PBS).
Thanks
SF
I have a stock conc of 100mg/ml of Rnase. Does anyone know how much Rnase to add for cell cycle analysis? I have about 100000 cells. Also, can teh RNAse be diluted in the FACs buffer (1%FBS in PBS).
Thanks
SF
I use about 100 ug/ml. I usually resuspend in 100 uL of Rnase solution
-canotto-