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Solutions for Collection and Culture of Dust Mites - appreciate any protienal ideas (Mar/05/2010 )

I'm a undergraduate of bioengineering and planning to design a experiment to study dust mites.
Now ,it's still at the very beginning. we are considering the Solutions for Collection and Culture of Dust Mites

please provide any possible solutions if u have any idea or experience.
thank you very.

-seamania-

The paper at this link has some ideas in it. It seems they cultured the mites in 1:1 dead skin:yeast at 25 degrees C.

-bob1-

bob1 on Mar 10 2010, 07:31 AM said:

The paper at this link has some ideas in it. It seems they cultured the mites in 1:1 dead skin:yeast at 25 degrees C.
could u give me some more details on"cultured the mites in 1:1 dead skin:yeast at 25 degrees C"?
And the paper u give me has permission for downloading, it's inconvenient for me here to pay. If u can access to it ,could u plese upload it here? thank u.

-seamania-

I don't have free access to the journal either. I would presume that the 1:1 skin:yeast is a mix of skin scrapings and yeast done on a weight per weight basis in an incubator of some sort with controlled humidity.

You probably won't need much to grow mites, they are pretty small.

-bob1-

seamania on Mar 6 2010, 06:39 AM said:

I'm a undergraduate of bioengineering and planning to design a experiment to study dust mites.
Now ,it's still at the very beginning. we are considering the Solutions for Collection and Culture of Dust Mites

please provide any possible solutions if u have any idea or experience.
thank you very.

This paper may give you some ideas.
Attached File

-klinmed-

Thank bob1 and klinmed for great help.. :rolleyes:

-seamania-