Hematin - calculation (Mar/05/2010 )

Hi everyone,
I need help with weighting the hematin(um?)
I need to know how to weigh it so its concentration will bečíme 40µM?
Dimension is 20µ L.

Linear Formula:C34H33N4O5Fe
Molecular Weight:633.49

Thx!!!

koljai on Mar 5 2010, 12:08 PM said:

-dissolve hematin in 20 mm NaOH or DMSO
-centrifugation for 10 min at 15,000 rpm
-remove pellet
hematin concentration is estimated from absorbance at 385 nm (εm m = 58,400) in 100 mm NaOH and adjusted to (Shaklai N., Shviro Y., Rabizadeh E., Kirschner-Zilber I.(1985) Biochim. Biophys. Acta 821:355–366)