protein extraction from tissue, help - (Mar/03/2010 )

Hi there,

I need to extract proteins from a tiny muscle fibre. I'm tending to use the T-PER tissue protein extraction reagent. But I don't know how i could perform the homogenization. Obviously, a hand-hold glass homogenizer won't do since my muscle tissue is very small. Any suggestion on this plz? like sonication?

Many thx,
Jo

in what volume will you be extracting? there are some very small teflon-glass homogenizers.

you could even homogenize in a eppendorf tube.