measuring apoptosis - reduced cell proliferation due to apoptosis (Feb/28/2010 )

Hi,

My gene of interest results in reduced growth rate compared to non-transfced parental cell line. Furthermore, transient transfection of my gene resukts in enhanced cleaved PARP.

I want to study if teh reduced cell proliferation is due to apoptpsis, what would be teh best way to approach this?

I'm thinking of using Caspase Inhibitor Z-VAD-FMK. Has anyone used this? Also, this kit inhibits caspas but the user has to carry out standard apoptosis assay to measure the level of aopotosis. I;m not not what approach to take?

Any ideas would be welcomed.

you are right, an apoptosis assay kit would do. you could look at different stages of apoptosis also. There are different assays kits for these also.