U/ml to ug/ml - conversion (Feb/24/2010 )

Hello All,

New to the forum, but have gotten great info in the past when searching for something on google.


I have a heparin stock solution at 1000 U/ml. Is it possible to make a 50mg/ml working stock from this solution?

If so, what other info do i need for the calculation?

Thanks,
BC

BryanC on Feb 24 2010, 03:26 PM said:

You need to know how many U are in a mg, and calculate from there

Thanks.


So, if the Heparin salt is 180 U/mg, then the 1000 U/ml sol'n is at .180 mg/ml? Which means I would have to make a fresh 50 mg/ml stock from the heparin salt?

Guess Ill have to order some more heparin, because someone has put a basically empty bottle back into the cabinet without ordering more.

180 U/mg will give 1000U.ml^-1/180U.mg^-1= 5.556 mg/ml not 0.18mg/ml - you'll still have to make a fresh stock or add a larger volume to a dilution.

bob1 on Feb 24 2010, 06:08 PM said:

Thanks bob. I really rushed that calc. And man was I over thinking U/ml, U/mg, etc. Never had to deal with "units" before....

Being that it's for in situ hyb, i don't think I trust the cleanliness of someone else's solution anyway.