low serum media and FCS - (Feb/16/2010 )

Hi,
Is it necessary to use a collagen coated plate for the propagation of mammalian cells grown in low serum media????

thank you

-deva.kumari-

deva.kumari on Feb 16 2010, 01:14 PM said:

Hi,
Is it necessary to use a collagen coated plate for the propagation of mammalian cells grown in low serum media????

thank you

Only if the specific cells you are trying to grow have problems with adherence.

Rhombus

-rhombus-

thank you for your response...
i am trying to culture primary renal cells..the media contains 0.5% FBS..i have not handled renal cells before...
normally primaries have a problem in revival after freezing...so do i go on with normal flasks????
thank you

-deva.kumari-