molecular biology - (Feb/02/2010 )

i want to kow that how many basepair must be flanking a restriction site in order to restrict it with type 2 restriction enzymes and how many basepairs should be flanking a gene in order to clone it in an expression vector. does number of flanking basepairs matter

Have a look at the attached table, it should help.

Good one..
Or you can check this, if you think above is not authentic..
http://www.neb.com/nebecomm/tech_reference...nucleotides.asp