Blood volume of rats - (Jan/21/2010 )
Can anyone please tell me how much blood volume a rat has based on its weight? and how much of it can i withdraw while still experimenting (mid-way) without causing any serious hemodynamic instability? thanx
if you go to google and type: "blood volume in a rat?" several promising links appear.
This is generally the rule that our lab follows, and it seems to be the norm.
The 1-3-6 rule is generally followed, in that the average blood volume of most laboratory animals is 6% body weight (60 ml/kg). The most blood that can be expected from a terminal sacrifice is 3% body weight (30 ml/kg); and no more than 1% (10 ml/kg) body weight should be collected during any 2-week period from animals surviving the blood collection.
I forget where I found that rule. It had to be on an SOP of a University animal facility of some sort. Hope that helps out a bit.
Stacy
aimikins on Jan 22 2010, 02:57 AM said:
Stace on Jan 22 2010, 06:06 AM said:
The 1-3-6 rule is generally followed, in that the average blood volume of most laboratory animals is 6% body weight (60 ml/kg). The most blood that can be expected from a terminal sacrifice is 3% body weight (30 ml/kg); and no more than 1% (10 ml/kg) body weight should be collected during any 2-week period from animals surviving the blood collection.
I forget where I found that rule. It had to be on an SOP of a University animal facility of some sort. Hope that helps out a bit.
Stacy
Thanx alot guy.
well i think brat have many specific brat so you can't have an accurate figures.