culture medium sterilization - (Jan/15/2010 )

Hi:

I have to sterilizate a solution of DMEM with a polisacharide dissolved. The filtration (0,22uM) is not work. There is another method to sterilizate a culture medium?. Is posible pasteurizate the medium (60ºC 30 min) and after add the antibiotics, the L-glutamine and the serum?

Thanks
Iker

0.22 um filteration is sufficient, why are you worried?

What do you mean the 0.22 um filtration doesn't work? Will it not go thru the filter or is it not sterile? You could try filtering it successively thru a 0.8 then an 0.45 then an 0.22 filter.

Do you mean that polysaccharide clogs the filter?
If so, try preparing it in a sterile manner. If you got the polysaccharide from sigma then it comes sterile. Open it in a laminar flow and use sterile water do dissolve it. The just add it into the DMEM.