Chosing the right competent cells - (Jan/08/2010 )

Hi,

my friends told me some competent cells are recommended for specific plasmids. just wondering what competent cell is recommended for pEGFP or pFLAG plasmids? can TOP10 be used for all? why some people chose other competent cells like JM109 or DH5a? why not have a universal competent cell?

Top10 will work well.

"The good thing about standards is that there are so many to choose from."