Preparing ethidium bromide stock solution. - (Jan/05/2010 )
Hello, I need help preparing ethidium bromide stock solution. I have the powder but the concentration is supposed to be 10 ug/ml and I can't measure out that small of a volume plus the powder has a clumpy consistency which makes it hard to work with in the first place. Can someone tell me how to make this working solution?
Thank you
-spellberg56-
The usual stock solution concentration of EtBr is 10 mg/ml, or 1000x higher. Are you sure you need the lower concentration?
I would make this by first making a 10 mg/ml stock and diluting 1000x (assuming that this is what you really need).
-phage434-
By default,
EtBr is actually 10mg per ml
we used to convert it to .5 microgram per ml
-Mazhar Hussain-