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Preparing ethidium bromide stock solution. - (Jan/05/2010 )

Hello, I need help preparing ethidium bromide stock solution. I have the powder but the concentration is supposed to be 10 ug/ml and I can't measure out that small of a volume plus the powder has a clumpy consistency which makes it hard to work with in the first place. Can someone tell me how to make this working solution?

Thank you

-spellberg56-

The usual stock solution concentration of EtBr is 10 mg/ml, or 1000x higher. Are you sure you need the lower concentration?
I would make this by first making a 10 mg/ml stock and diluting 1000x (assuming that this is what you really need).

-phage434-

By default,
EtBr is actually 10mg per ml
we used to convert it to .5 microgram per ml

-Mazhar Hussain-