membrane type of cell culture filtration - (Jan/05/2010 )

hi
there are different kinds of membranes in 0.2 micrometer syringe filters, which one is recommanded for filterind cell culture solutions and media?
regards

Hi
I think Milipore is good

good luck

Sara... use one with a low protein binding capability - you don't want to remove the precious proteins and amino-acids from your medium.

sara.r on Jan 5 2010, 02:31 PM said:

We generally use Cellulose Acetate memrane filters. As bob1 correctly states, any membrane that has low protein binding, as you do not want to lose precious growth factors.

I do however find it strange that when media, FCS, PEN/STEP, NEAA etc all come sterile...why would you want to filter it.

If you are adding drugs/compounds into your TC media, I have always found it best to make them up in a concentrated solution....then filter sterilise that.

Hope this is useful.

Kindest regards.

Uncle rhombus