PCR troubleshooting - two band after agrose electophoresis (Jan/03/2010 )
hi all:
im new in molecular bilogy, i had amplified DNA product by primer that has high G,C content designated by garovic et al.2003. to get 107bp, i run the agrose gel 2.5%, then i got two band in the expected site,,,why two bands? plz help
max,,
-maxmix-
Hi
Most probably You have a primer dimers.
Do You see one of the bands on no template control?
-Michaelro-
hi max,
can you attach your picture?
-adrian kohsf-
Michaelro on Jan 4 2010, 12:15 AM said:
Hi
Most probably You have a primer dimers.
Do You see one of the bands on no template control?
Most probably You have a primer dimers.
Do You see one of the bands on no template control?
-maxmix-
adrian kohsf on Jan 4 2010, 12:40 AM said:
hi max,
can you attach your picture?
can you attach your picture?
-maxmix-