Sequence Specific Oligonucleotide Analysis, Interpretation - (Dec/25/2009 )
I am bit confuse about Sequence Specific Oligonucleotide Analysis...
Principle Says,
By "Mismatched" heteroduplex formation, it will cause its speed on the Acryamide gel slow as compared to normal band...
I have to Ask following questions,
1. In such analysis which PCR variant is used for DNA amplification?
2. If Fragment "mismatched" used to be slow as shown in lane 1 and 4 of following figure, then why this didn't heppen in lane 2 and 5? (I think this band was to appear beside the upper band)
Thank You very much in advance for paying keen attention on this problem....
-Mazhar Hussain-
1. No full paper, hard to tell.
2. This is not mismatch, this is deletion.
-adrian kohsf-
adrian kohsf on Dec 27 2009, 10:40 AM said:
1. No full paper, hard to tell.
2. This is not mismatch, this is deletion.
2. This is not mismatch, this is deletion.
Hi,
I have uploaded now...
-Mazhar Hussain-