RBC hemolysis - (Dec/15/2009 )
thats right bachai, i have tried tail bleed but could not get blood..i need at least 200microL blood.
bachai on Dec 22 2009, 06:47 PM said:
I often had hemolysis on eye bleeding. And the worst was I made a number of poor creatures blind after serial bleeds.
Then I had much less hemolysis and spent less time with tail bleeding. Prewarm mice 3-5min before bleeding. No anaesthesia required by my institution animal ethics. Use a mouse strainer to fix the animal except the tail. I got ~50uL serum per mouse this way every fortnight. Of course you could get more but the animal ethics may not allow you to. In general I believe tail bleeding is less stressful both for me and mice.
Then I had much less hemolysis and spent less time with tail bleeding. Prewarm mice 3-5min before bleeding. No anaesthesia required by my institution animal ethics. Use a mouse strainer to fix the animal except the tail. I got ~50uL serum per mouse this way every fortnight. Of course you could get more but the animal ethics may not allow you to. In general I believe tail bleeding is less stressful both for me and mice.
-ujla80-