SDS-Page and DMSO - (Dec/14/2009 )

Hi,

I would like to incubate a protein complexe in 70-90% DMSO and then separate it with SDS-PAGE. Somebody knows if DMSO can cause troubleshooting during electrophoresis?

Thank you for your answer.

Hi Baeinoix,

I do not think you should have difficulty with electrophoresis. Pierce makes a kit (SDS-PAGE sample prep kit 89888) that uses DMSO in a protein binding buffer. The troubleshooting section says to increase to >60% to improve efficiency of binding. here is the link:

http://www.piercenet.com/files/1728dh5.pdf

regards,

lab rat

Thank you for your answer but it seems that the DMSO is removed in the kit during wash step. I think I will remove DMSO by SpeedVac my samples...