cell adhesion assay - collagen (Dec/11/2009 )

Hi,

I plan to do a cell adhesion assay (non flurescent) soon for my parental cell line and cell line transfected with gene of interest. I have no experience doing this and thus all suggestions would be welcomed.

I have recently ordered collagen but can someone share with me on how to pre treat the plates with collagen and ths standard procedure to carry out the assay.

check out Cell-Cell Interactions Methods and Protocols and Adhesion_Protein_Protocols from Methods in Molecular Biology.. you'll get all varieties of cell adhesion assays