how to check the quality of DNA? - (Dec/08/2009 )

Hi,

i have a problem with the quality of my DNA. This would be important for doing qPCR (background).
how can i check the quality? Any suggestion?

greetz,

susan

-susanna-

Hi susanna,

Just run an agarose gel (1%) with a 25 ng or so of gDNA. Electrophorese and stain with EtBr. A smear will indicate degraded gDNA.

regards,

lab rat

-lab rat-

Hi
You could also record a UV spectrum....to get an idea of protein contamination..OD260/280 between 1.7 and 2 would mean that DNA quality is good.
All the best

-arc1-

arc1 on Dec 12 2009, 03:40 AM said:

Hi
You could also record a UV spectrum....to get an idea of protein contamination..OD260/280 between 1.7 and 2 would mean that DNA quality is good.
All the best

thanks

-susanna-

so which device do you suggest for UV spectrum?

-susanna-