About tissue culture 96 well plates - (Dec/06/2009 )

I have tissue culture plates with flat bottom for suspension culture from Jet Biofil. Can i use these plates for adherent cancer cell lines or is there any treatment that i should do to make plates adherent so cells will adhere.

many adherent cell lines can be cultured in ordinary plates without any need to special treatment of the plate.

coat the wells in gelatin or poly-L-lysine.

mtrnbh on Dec 6 2009, 11:30 PM said:

Thanks for your reply and i am going to use HEPG2 cell line can it be ued. And the plates are made up fron poly styrene and can it be used for adherent cell lines.

Actually cell culture plates are specially charged polystrene - so they should work fine, though the charge may not be right for culture.