Page or HPLC primers - What is the difference (Nov/24/2009 )
The title says it all. Embarassing to admit I still don't know that, but, hey better to learn than stay an idiot, right?
So what is the difference between these 2? And what is the Purest? ![]()
My primers are between 30 and 37bp with a Biotin at the 5' end.
Here is what Invitrogen says:
HPLC: Guarantees highly purified primer required in some applications (=>85% full-length).
PAGE: Provides the highest percentage of full-length oligos (>=85%) required for certain demanding applications such as mutagenesis or adapter production
huh?
Oh and maybe I should have said that it's urgent because I need to place a huge primer order (almost 200) asap.
OKeeeeeeeeeeee, looks like I'm not the only ignorant around here
So I give you my findings. HPLC and PAGE don't use the same tecnhique to separate according to lenght/molecular weight/charge and from what I gathered, HPLC's purity is between 90-97%, wherease PAGE is between 95-99%.
They cost the same (at least with Sigma), so I doubt the difference is big anyway.
Ahhh don't I love talking to myself ![]()