sequencing the reverse strand - (Nov/24/2009 )
Hi,
just to make sure: if I want to bisulphite sequence the reverse strand of my piece of DNA I can just design the primers according to the reverse complement of my forward strand, right?
...
-ForEpi-
yes the reverse complement of the forward strand PRIOR to mock bisulphite conversion.
ie: take your original genomic DNA sequence, reverse complement and THEN mock bisulphite treat using find and replace in word.
-methylnick-
Cheers Nick.
-ForEpi-