This sumo sequence correct? - sumo (Nov/24/2009 )
HI,
I want to construct a sumo vector, and got the sumo sequence in the paper"SUMO Fusion Technology for Enhanced Protein Production in Prokaryotic and Eukaryotic Expression Systems", but am not sure the accuracy of this sequence
The DNA and protein sequence listed as below
TCCCTGCAGGACTCAGAAGTCAATCAAGAAGCTAAGCCAGAGGTCAAGCCAGAAGTCAAGCCTGAGACTCACATCAATTT
AAAGGTGTCCGA
TGGATCTTCAGAGATCTTCTTCAAGATCAAAAAGACCACTCCTTTAAGAAGGCTGATGGAAGCGTTCGCTAAAAGACAGG
GTAAGGAAATGG
ACTCCTTAAGATTCTTGTACGACGGTATTAGAATTCAAGCTGATCAGGCCCCTGAAGATTTGGACATGGAGGATAACGAT
ATTATTGAGGCT
CACCGCGAACAGATTGGAGGT
SLQDS EVNQE AKPEV KPEVK PETHI NLKVS DGSSE IFFKI KKTTP LRRLM EAFAK RQGKE MDSLR FLYDG IRIQA
DQAPE DLDME DNDII EAHRE QIGG
Could anyone who has the information please give me a confirmation or revision? Thanks very much!
Chenjun
Go to this site, choose nucleotide, the on the nucleotide page chose BLAST and follow the instructions.
Your seq. is part of of the Expression vector pASHSUL.
Use BLAST to see it