RNA gel electrohoresis - (Nov/18/2009 )
Kindly let me know as to why do we need to use both formamide as well as formaldehyde during the RNA separation by agarose gel electrophoresis-I know that they are used as denaturants-but why both and why not just one of them?
thanks in advance
-ashpra-
depends on which protocol you are following.
formaldehyde is used in the gel.
you use either formaldehyde or formamide in the sample buffer.
-mdfenko-