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RNA gel electrohoresis - (Nov/18/2009 )

Kindly let me know as to why do we need to use both formamide as well as formaldehyde during the RNA separation by agarose gel electrophoresis-I know that they are used as denaturants-but why both and why not just one of them?
thanks in advance

-ashpra-

depends on which protocol you are following.

formaldehyde is used in the gel.

you use either formaldehyde or formamide in the sample buffer.

-mdfenko-