protocol of glycine elution gradient - (Nov/18/2009 )
Hello all, I am going to elute my protein complex from the antibody covalently binding to the proteinA beads. one protocol recommended to use glycine elution gradient. I am not quite familiar with this techniques, does anybody have a protocol? thanks 
-shm267-
http://www.piercenet.com/Proteomics/browse...6BF3E92#buffers
might help..
or tere are many apication notes in pharmacia site... u just need to give a gradient of the glycine buffer.. depending on the affinity, the protein will elute
-Pradeep Iyer-
Pradeep Iyer on Nov 18 2009, 01:04 AM said:
http://www.piercenet.com/Proteomics/browse...6BF3E92#buffers
might help..
or tere are many apication notes in pharmacia site... u just need to give a gradient of the glycine buffer.. depending on the affinity, the protein will elute
might help..
or tere are many apication notes in pharmacia site... u just need to give a gradient of the glycine buffer.. depending on the affinity, the protein will elute
thank you for your help.
-shm267-