5-azacytidine - calculation (Nov/06/2009 )

Hi,

I have to make up 500um stock concentration of 5-azacytidine. Now, its come in a lyophilized form and it says (50X), FW 244.21. Can someone assist me in teh caculations pls.

SF_HK on Nov 7 2009, 01:11 PM said:

Sorry, I'll clarify myself further.

The bottle says 50X and the manual says: "add 10ml sterile culture medium. The working concentration is 10 μM, so the 50X stock is 500 μM"

After I add the 10ml autoclaved PBS, do I hate to filter sterilize it further? I will ahve to aliquot the stock at keep in -20deg

well i think autoclaving is enough.. but if u still feel the need to fileter it u can do so before or after... but in the laminar to maintain the asceptic conditions!!!
Also irs better to aliquot and store it in the mentioned temperature (if its -20 then tere) as repeated freeze thaw nmight also disturb the stability of the molecule!!
Best luck!!!