why gel is not running in straight line?? - (Nov/04/2009 )
adrian kohsf on Nov 4 2009, 10:18 PM said:
this used to happen to me on the first and last well, I also cant figure it out why... maybe too high voltage is the culprit. My solution: i ignore the 1st and last well when i run my page gel...
this happens because the edges run cooler than the central portion of the gel.
-mdfenko-
The temp of the gel can make a difference. Do you circulate your running buffer? If the tank is small, you can stick it in an ice bucket and it will be fine. If it's large, you can set it on a magnetic stirrer with a stir bar underneath and run it at a lower voltage, or use an attachment for a circulating cold water bath.
-lab rat-
mdfenko on Nov 6 2009, 01:06 AM said:
adrian kohsf on Nov 4 2009, 10:18 PM said:
this used to happen to me on the first and last well, I also cant figure it out why... maybe too high voltage is the culprit. My solution: i ignore the 1st and last well when i run my page gel...
this happens because the edges run cooler than the central portion of the gel.
Oh....I didn't know that....haha... thank you for the enlightenment.
-adrian kohsf-
Plus, avoid loading too much sample into one gel. You can always divide your sample volume into half and load them into two wells.
-jiajia1987-