Protocol Online logo
Top : New Forum Archives (2009-): : Microbiology

Universal 16s rRNA primers needed - (Oct/30/2009 )

Hi all,

I need your help.

I need a universal 16s rRNA primers which is already made as commercials.

Do you know which company have this pre-made Universal 16s rRNA primers? (this is not custom made)

Thank you

-lojinsky-

Why don't you just pull them out of a publication?

Here's a link. http://aem.asm.org/cgi/content/abstract/AEM.02220-08v1

Regards,
lab rat

-lab rat-

See this page:
http://openwetware.org/wiki/Bacterial_species_identification

-phage434-

hello
try following primers known as 27F and 1492R, they amplify a long 1.5 kb almost all of 16S gene, they work perfectly. do tell if u need short segments

27f AGA GTT TGA TCC TGG CTC AG

1492 TAC GGT TAC CTT GTT ACG ACT T

-microbes-