How Best to Clean the Sonicator Tip Between Samples? - (Oct/28/2009 )
Hi! Can someone tell me the best way to clean the tip of the sonicator between lysate samples? I want to make absolutely sure that I don't have any protein carryover, but I don't want to do anything to the sonicator that might compromise my samples. Any advice would be appreciated! 
What about disposable probe covers? They make them for thermometers, but maybe they will work for sonicators.
http://www.fishersci.com/wps/portal/ITEMDE...uctsItemsFlag=Y
http://www.labsafety.com/Welch-Allyn-SureT...160/?CID=9PP001
http://www.allegromedical.com/diagnostic-p...11124bc743356ff
I clean it between samples with the same buffer i am using to sonicate. And after that with ethanol
Envennom on Oct 28 2009, 11:43 PM said:
i just wipe the tip with 70% EtOH.
Envennom on Oct 28 2009, 11:13 AM said:
Envennom,
wiping with ethanol or sonication of ethanol in a beaker is an effective way.
Alternatively, you could sonicate your samples in a sample vial, such as an Eppendorf 1.5mL. Hielscher offers a product called VialTweeter for this. This allows for the sonication of up to eight samples at a time. No wetted probe --> no cross-contamination, no cleaning/rinsing/wiping.
Save time and focus on your research instead of performing tedious cleaning tasks
Hielscher_Ultrasonics on Nov 23 2009, 08:56 AM said:
Envennom on Oct 28 2009, 11:13 AM said:
Envennom,
wiping with ethanol or sonication of ethanol in a beaker is an effective way.
Alternatively, you could sonicate your samples in a sample vial, such as an Eppendorf 1.5mL. Hielscher offers a product called VialTweeter for this. This allows for the sonication of up to eight samples at a time. No wetted probe --> no cross-contamination, no cleaning/rinsing/wiping.
Save time and focus on your research instead of performing tedious cleaning tasks
Would cleaning with autoclaved water be sufficient to avoid cross contamination?
https://www.cascadehealthcaresolutions.com/alaris-disposable-probe-cover-p/alrp850a.htm