Intra-assay reproducibility - The basics (Oct/21/2009 )

Hello,
I've just joined this forum. I'm a translator, not a scientist, so forgive my ignorance.

I'm working on a translation for an immunochromatographic slide test kit for TB diagnosis, and I'm not clear what an intra-assay reproducibility test is when applied to such a test kit. Would it actually involve applying aliquots of the sample solution to the same test strip several times in succession? And what would be the definition of a replicate and a test run?

Thanks in advance,
S.

-amatory_rota-

A replicate is the same sample tested more than once, though there are biological and technical replicates that are possible as different things. This recent discussion should show you the difference:

http://www.protocol-online.org/forums/inde...showtopic=10920

A test run is either a run to test the protocol (i.e. with known positive and negative samples) or just running some samples through the protocol as you would normally to test them to see if they are positive or negative (as in "I'm testing my samples today").

Intra assay reproducibility is ensuring that you get the same result with the same sample. I haven't used any of these sorts of kits so I don't know how it applies to a TB test.

-bob1-

bob1 on Oct 21 2009, 03:30 PM said:

Thanks. So in my case, it looks as though it would be testing that the positive control, for example, produces the same result over multiple test strips, rather than that a single test strip gives the same results several times (which I should think is impossible given the nature of the test strips).

S.

-amatory_rota-