pulse chase experiment - (Oct/15/2009 )

hiiiii,
can someone tell me the complete protocol for pulse chase experiment??

sssss on Oct 15 2009, 01:17 PM said:

What kind of cells do you want to use and which isotope for what approach?
Lg Jana

hiiiiii,
I will transiently transfect the adherent cells and check the protein stability.i will use S-35(met) isotope.