Cycloheximide Concentration - (Oct/07/2009 )

Hi,

I'm trying to inhibit a stock strain of wild-type Saccharomyces with cycloheximide.....but am unsure what concentration to use.


Does anybody know of a resource i can use, or what concentration is commonly used?

Thanks

Mattbat

Matt - the levels I've used are ~ 0.5g/liter.

Saw (old) paper (http://jb.asm.org/cgi/content/abstract/86/5/956 ) reporting apparent cycloheximide induction of petite variant of related species.